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Core Research Facilities and Resources
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Last Updated 8/19/2016 5:09 AM
RNAi Core
Director(s): Sheila Stewart
Contact: Tammi Vickery
Phone: 362-7437
Email: rnairequest@genome.wustl.edu
Web:
Campus: Medical Campus Campus Box: 8228
School/Unit: Medicine
Academic Department: Cell Biology And Physiology
*Please contact this Core directly for physical location
Core Location
Building Name: Genome Center
Room Number: 4th floor
Core Summary
The Core supplies viral-based RNAi constructs targeting every gene in the human and mouse genomes. The DNA is transfection quality which can be delivered directly to target cells or used to create infectious lentiviral constructs. Constructs are ordered by gene and in general there are 5 constructs per gene. On average two or more constructs will knockdown their target genes by greater than 75%.
Access
Service available to:
Washington University only

Priority service for:
Specific groups, centers or departments – see Additional Information for details

Additional Information:
Affiliation

  • N/A
Core Details

The Core supplies viral-based RNAi constructs targeting every gene in the human and mouse genomes.  The DNA is transfection quality which can be delivered directly to target cells or used to create infectious lentiviral constructs.

Constructs are ordered by gene and in general there are 5 constructs per gene.  On average two or more constructs will knockdown their target genes by greater than 75%.


RNAi DNA Request Procedure: 

The Gene Symbol and Gene ID of interest should be sent via email to the following address:

rnairequest@genome.wustl.edu

Please specify whether you would like human or mouse clones.  Upon receipt of the request, we will send you an official cost estimate for the distribution of the clones. Estimates can be accepted via email with a simple reply from you, by fax, or in person.  Instructions will be sent with the cost estimate.

Once the estimate has been officially accepted, DNA will be provided to you.  For each gene request, all available hairpin constructs that target the gene of interest will be supplied in a 96 well format.  Each sample will consist of ten microliters of sample.  In addition, a template in the form of a table will be supplied with each request.  This template will list the locations of each construct.  Control wells will be provided in the following manner.  Well A1 of the container will be left empty as the negative control.  Wells A2 through A5 will contain a single construct that targets RFP, GFP, LacZ, and Luciferase, respectively. 

The quality of each sample will be confirmed by visualization on an agarose gel.  The human constructs have been sequenced verified. Mouse constructs can be sequenced verified for an additional fee.  If the research performed with these clones leads to a publication, the requestor is required to acknowledge the TRC, CDI, and the TGI. 

Services

  • Deliver transfection quality DNA. The core can also provide advice on lentiviral production and supply packaging plasmids.
Equipment

  • N/A
Pricing - subject to verification from the Core Facility.

In general there are 5 constructs per gene. Each construct costs $30.00, so each gene will cost approximately $150.00. The human constructs have been sequenced verified, but the mouse constructs will not be sequenced verified.  If you would like sequence verification, an sequencing fee will be charged.  Please contact us for current sequencing pricing.



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