The Hybridoma Center / Monoclonal Antibody Development Core is a specialized cell culture facility that assists investigators in the generation of monoclonal antibody producing B cell hybridomas. Mouse and Armenian hamster models are utilized to generate antigen reactive monoclonal antibodies. Open to all.
Core Description
The Hybridoma Center / Monoclonal Antibody Development Core assists investigators in the custom generation of monoclonal antibody producing B cell hybridomas. Guidance is provided in the selection of appropriate model species, development of immunization strategies, screening protocols and methods for antibody production. Immunization services are available for mouse and Armenian hamster models. Once sufficient antibody titers are identified from immune animals, splenocytes are fused with a murine myeloma and cell cultures are maintained in the Core facility. Hybridoma culture supernatants are harvested and screened for the presence of antigen reactive antibody. The Hybridoma Center can provide ELISA based screening, however, more sophisticated assays are performed by individual investigators. Hybridomas secreting antigen-specific antibodies are subcloned by limiting dilution to establish a stable cell line secreting a unique antibody with defined specificity and isotype. Mycoplasma screening and mAb isotype services are available. Hybridoma stabilization and cell line optimization are provided for large scale expansion. Cell banks are generated and are returned to the investigator or may be stored long term within the Core facility. All materials generated remain the property of the investigator.
Access
Service available to All entities, including for-profit organizations.
Priority service for All entities, including for-profit organizations.
Additional information:
Contracted services are open to non-University organizations on a fee for service basis. In vivo immunization services and in vitro cell culture services are provided.
Services
- Immunization of mice (wild type, genetically engineered or autoimmune) or Armenian hamsters
- Conventional and genetic hydrodynamic immunization
- B cell fusion for monoclonal antibody development
- ELISA-based supernatant screening
- hybridoma subcloning
- cryopreservation, isotype analysis
- cell line optimization and banking
- consultation for immunization methods and screening strategies
Equipment
- N/A
Pricing
Please contact this core directly for pricing information.