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Last Updated 6/27/2017 6:48 AM
Washington University Mouse Embryonic Stem Cell Core
Director(s): Benjamin D. Humphreys MD, PhD
Contact: Aileen Lu
Phone: 314-747-9707
Email: lu633@wustl.edu
Web:
Campus: Medical Campus Campus Box: 8126
School/Unit: Medicine
Academic Department: School Of Medicine
*Please contact this Core directly for physical location
Core Location
Building Name: Wohl Clinic
Room Number: 9946
Core Summary
This core assists investigators with the generation of manipulated murine ES cells used to make sophisticated mouse models of human diseases.
Access
Service available to:
Washington University and other non-profit organizations

Priority service for:
Specific groups, centers or departments – see Additional Information for details

Additional Information:
Affiliation

  • Departments of Medicine; Developmental Biology; Cell Biology & Physiology
Core Details
The Murine Embryonic Stem Cell Core has been created to help you make mutations in mouse embryonic stem cells.  The core has several missions including: development of state-of-the art reagents for the production of targeted mutations in embryonic stem cells, the creation of quality-controlled embryonic stem cell lines, and the teaching of methods for embryonic stem cell culture and manipulation.  The core provides seven different embryonic stem cell lines for the WUMS research community.  In addition, this core provides a teaching service, so that 'hands-on' production of targeted ES clones can be learned.  Finally, the core will arrange additional services for your ES clones such as karyotyping, MAP testing, and blastocyst injections.  By providing a comprehensive service, we hope to facilitate the production of gain-of-function and loss-of-function mouse models for our faculty.​
Services

  • Primary ES cell Transfections;Secondary Transfections (utilizing either cre or flp recombinase);Expansions of ES clones; Plasmid distribution; Additional ES cell testing preparations and arrangements (mycoplasma, MAP, karyotyping, blastocyst microinjections); Cryopreservation storage of ES clones; Availability of wild-type ES cell lines
Equipment

  • All equipment associated with a tissue culture facility; Bio-Safety hoods; Two tissue culture incubators; Essential equipment for transfection, clone plucking and clone preservation; Appropriate computer support for administration and record keeping
Pricing - subject to verification from the Core Facility.
Please refer to the core web site under 'Services' for detailed service costs.

Additional Keywords
mouse embryonic stem cell