The Animal Model Research Core provides breeding and colony maintenance of rodent models used for nutrition-related research (e.g., animals with lipid abnormalities, atherosclerosis, obesity and diabetes), genotyping, histological analyses, biochemical measurements (plasma lipids, glucose, and hormones) and body composition analyses.

Core Description

The Animal Model Research Core facilitates research related to nutrition and obesity. The Core provides cost-effective specialized assays relevant to the nutrition and obesity mission of the NORC, access to well-characterized mouse models used in nutrition research, services critical for evaluation of appropriate mouse phenotypes, and training required for testing hypotheses related to nutrition and obesity in animal models.

Access

Service available to All entities, including for-profit organizations.

Priority service for No distinctions.

Additional information:

Services available to members of the Nutrition Obesity Research Center (NORC). Applications to the NORC can be found at https://gns.wustl.edu/research/nutrition-obesity-research-center/membership.

Services

  • Providing genetically engineered mice from small breeding colonies for nutrition-related research
  • Performing biochemical assays of serum
  • Performing biochemical assays of tissues
  • Providing facilities and reagents for assaying mRNAs of genes relevant to nutrition and obesity research
  • Maintaining facilities for and providing instruction in assessing atherosclerosis in mice
  • Assaying metabolic parameters including metabolic rate and food intake
  • Determining blood pressure
  • Glucose tolerance and insulin tolerance testing
  • Hyperinsulinemic/euglycemic clamp procedure
  • Determination of body composition
  • Training/consultation services dealing with establishing mouse colonies and phenotyping animals
  • Immunocytochemistry of mouse tissues

Equipment

  • ECHO MRI machine with support equipment for body composition determination
  • several conventional PCR machines
  • a Leica CM 1850 Cryostat for preparing tissue sections
  • an ABI prism 7000 quantitative RT-PCR machine for quantifying message levels by RT-PCR
  • Oxymax small animal indirect calorimetry systems
  • a Columbus Instruments NIBP-8 Non-Invasive (tail cuff) blood pressure monitor for rodents interfaced with a computer
  • 6 PA-C10 transmitters and receivers for telemetry determination of blood pressure
  • 6 TA10TA-F20 transmitters for core temperature determination
  • 8 metabolic cages for determination of food intake and collection of excretions
  • a high resolution digital camera attached to a microscope and integrated with a computerized image processing system for quantifying atherosclerotic lesions
  • a Synergy 4 Multi-mode Micro Optical plate reader adapted for high throughput determination of serum chemistries
  • an Amersham Pharmacia AKTA FPLC apparatus used to assay mouse lipoproteins by size exclusion
  • an Alpha Innotech FluorChem 9800 system
  • a variety of infusion pumps
  • several types of centrifuges
  • a variety of microscopes
  • a Kodak In-Vivo Multispectral Imaging System FX with a dedicated PC and the appropriate software
  • TSE phenomaster with eight cages for metabolic assessment, activity, food, drink and body weight determination. Additionally, four TSE cages are available for urine and feces collection and determination; other appropriate support equipment.

Pricing

Please contact this core directly for pricing information.


AFFILIATIONS
Diabetes Research and Training Center (DRTC)
Nutrition Obesity Research Center (NORC)