In vivo brain microdialysis in mouse and rat models.
Microdialysis has been used for over 30 years to sample molecules within the extracellular space of a tissue, including brain and muscle. One advantage of the technique is that it can be performed in awake, behaving animals so that longitudinal measurements can be performed under physiological conditions. Brain ISF can be sampled hourly in living mice using microdialysis. The technique not only determines the magnitude of change in the target, but also the kinetics of that change. The Core was originally designed to test compounds and proteins for their ability to alter brain interstitial fluid (ISF) amyloid-beta (Abeta) levels. In addition to Abeta, we are able to detect a variety of molecules and proteins within the brain ISF, including small molecules such as glutamate and urea as well as peptides such as orexin. Using a new 1,000 kDa MWCO microdialysis probe, we are now able to detect very large proteins within the ISF such as apolipoprotein E, soluble APP, and SOD1. Of course we are always interested in measuring other targets if necessary. We are equipped to perform microdialysis in both mice and rats.
Service available to All entities, including for-profit organizations.
Priority service for No distinctions.
- In vivo microdialysis
- CSF isolation from mice and rats
- PK/PD studies
- In vivo microdialysis rigs
Please contact this core directly for pricing information.
Hope Center for Neurological Disorders (HCND)
Hope Center Program on Protein Aggregation and Neurodegeneration (HPAN)