The Proteomics Core was established in 2002 to support basic and translational research studies and programs with current proteomic methods and expertise. The Core is supported, in part, by the Siteman Comprehensive Cancer Center, Institute of Clinical and Translational Sciences (ICTS), and the NIH / NIGMS Biomedical Mass Spectrometry Resource. The ICTS offers funding for usage through the Just-In-Time Core usage funding program.

Core Description

The Proteomics Core provides services for discovery and targeted proteomics using tissues, cells, and biological fluids (e.g. plasma/serum, cerebrospinal fluid and urine). The Core performs small (≤ 20 samples) and large-scale studies with ~100 clinical samples.

Sub-proteome (e.g. kinome, exosomes and protein complexes) analysis is performed using current enrichment or fractionation protocols. Both label-free and isobaric peptide labeling methods are available for relative quantification proteins and protein modifications (e.g. phosphorylation) to a depth of ≥ 10,000 and ≥ 37,000 sites, respectively. Absolute quantification (copies/cell) is performed using synthetic signature peptides with stable isotope dilution mass spectrometry. The Core hosts ~1000 labeled synthetic peptides for pan-kinome measurements (361 kinases) and metabolic pathways (67 enzymes). These assays have been validated under the guidelines of the National Cancer Institute/Clinical Proteomics Tumor Analysis Consortium Proteomic experimental design, data processing, analysis and interpretation are offered by the faculty and staff

Dr. R. Reid Townsend (rtownnsend@wustl.edu, 314-362-7709),

Dr. Qiang (Tim) Zhang, (zhang.qiang@wustl.edu, 314-362-9134),

Dr. Robert Sprung, (robert.sprung@wustl.edu, 314-362-9134).

Access

Service available to All entities, including for-profit organizations.

Priority service for No distinctions.

Additional information:

Contact the Core Lab Manager, Jim Malone at jmalone@wustl.edu (314-362-9122) to schedule an initial meeting or obtain additional information.

Services

  • Multi-affinity chromatography - selective removal of high abundance proteins in biological fluids
  • Exosome enrichment using an affinity method
  • Quantitative, label-free proteomics (LC-MS)
  • Customized sample preparation for a wide range of sample types and protein compartments
  • High-resolution nano-LC-MS
  • Data quality assessment, processing, analysis and assistance with interpretation
  • Development of customized assays for high-precision absolute protein quantification

Equipment

  • Q-Exactive™ Orbitrap™ PLUS interfaced to an EASY-Spray™ nLC 1000 – (Thermo Scientific)
  • Q-Exactive™ Orbitrap interfaced to a Dionex Ultimate RSLnano LC – (Thermo Scientific)
  • Q-Exactive™ Orbitrap interfaced to an EASY-Spray™ nLC 1000 – (Thermo Scientific)
  • UltrafleXtreme™ MALDI-TOF/TOF System – (Bruker Daltronics)
  • Biomek NXp Automated liquid handling robot - (Beckman Coulter)
  • 1260 Infinity II Bio-Inert LC System – (Agilent Technologies)

Pricing

Pricing is subject to core verification

Current pricing and quotations are available upon request

Please contact Jim Malone.


AFFILIATIONS
Institute of Clinical and Translational Sciences (ICTS)
Siteman Cancer Center (SCC)
WU NCATS Mass Spectrometry Research Resource
NIH-NCI-Proteomics Characterization Centers