Animal Care and Use

Oocyte Harvesting in Xenopus laevis

Approved and Adopted: Prior to 2015
Last Revised: January 2024

Xenopus frog oocytes are used for studies of molecular biology, embryology and biochemistry and are often obtained by laparotomy. Frogs in good health may undergo a maximum of six (6) laparotomies for the surgical harvest of oocytes. Each laparotomy must be followed by a minimum four week rest period. The first five procedures may be survival surgeries. The sixth surgery must be non-survival and the animal euthanized prior to recovery from anesthesia. The incision sites must be alternated left to right between each surgery.