The mission of the GESC is to empower its users with access to the most up-to-date technologies in the fields of gene editing and stem cells and enable the creation of research models best fit for the unique need of each lab.
Using Clustered, Regularly Interspaced Short Palindromic Repeats (CRISPR) technologies as its core technologies, the GESC creates investigator-specified targeted modifications in cancer and stem cell lines, designs and validates targeting reagents for animal model generation and provides various genotyping assistances. On the stem cell front, the GESC creates induced pluripotent stem cells (iPSCs) from patient samples and differentiates iPSCs into disease-relevant cell types, supporting research in personalized medicine.
Service available to All entities, including for-profit organizations.
Priority service for Washington University and other non-profit organizations.
- gRNA and/or donor design, assembly and validation in cultured cells for investigators who are making an animal model or wish to do cell line engineering themselves
- Cancer cell line engineering-to introduce investigator-specified genetic modifications, including knockout and knockin, into the genome of a cancer cell line chosen by the investigator
- Stem cell engineering--to introduce investigator-specified genetic modifications, including knockout and knockin, into the genome of an iPS or human ES cell line chosen by the investigator
- NGS-based genotyping for cell lines and tissues from investigators who are making an animal model or wish to do cell line engineering themselves
- Patient fibroblasts (from skin biopsy), renal epithelial cells (from urine sample), and PBMCs (from whole blood) isolation, expansion and banking
- Reprogramming patient cells from above to iPSCs and characterization of the iPSCs, such as karyotyping, pluripotency staining and embryoid body formation and germ layer staining
- Pluripotency staining of iPSCs and hESCs
- Embryoid body formation of iPSCs and hESCs
- Differentiation of iPSCs or hESCs to neuronal lineages, cardiomyocytes, hematopoietic stem cells, macrophages, etc.
- Hands-on, on-demand training on iPSC culturing
- NGS-based STR profiling for cell line authentication
- Mycoplasma testing
- CRIPSR library design and construction and screening and analysis
- General molecular biology assistance
- Biomek FX
- Appliedbiosystems Quant Studio3 Real-Time PCR system
- Lonza 4D X-unit Nucleofector
- Sony Cell Sorter SH800
- Cell Metric Imager (Solentim)
- Biotek Synergy HTX Multi-mode Reader
- Biotek MultiFlo Washer and Dispenser
- Tecan Fluent liquid handler
- IncuCyte Imager S3
Please contact this core directly for pricing information.
Alzheimer's Disease Research Center (ADRC)
Hope Center for Neurological Disorders (HCND)
Institute of Clinical and Translational Sciences (ICTS)
Siteman Cancer Center (SCC)
McDonnell Genome Institute