The Genome Technology Access Center (GTAC) provides DNA/RNA/Protein analysis resources for researchers at Washington University and beyond. We currently provide NextGen sequencing (1 Illumina HiSeq3000, 3 Illumina HiSeq2500s, 2 Illumina MiSeqs), microarray (all formats) and quantitative PCR, including Fludigm arrays and single cell. We provide analysis services with allof these technologies.

Core Description

The Washington University Genome Technology Access Center (GTAC) provides microarray, Q-PCR, and next-generation sequencing (NGS) services to the Washington University community. The NGS core of the GTAC was established in the Center for Genome Sciences in mid-2008.  It is located in the Cortex building adjacent to the medical campus.  The core occupies approximately 2000 square feet and is currently staffed by fourteen scientists.  The NGS GTAC team involves itself in every step of sequencing research, including experimental design, DNA/RNA QC, library construction, sequencing, and analysis of next-generation sequence data.  The NGS core is equipped with one HiSeq3000, three HiSeq 2500, and two MiSeq Illumina sequencers.  The facility currently prepares Illumina sequencing libraries for RNA-seq, single-cell RNA-seq, ChIP-seq, targeted capture, whole exome capture and genomic sequencing. For DNA/RNA QC, the team utilizes a Qubit fluorometer, Agilent 2100 BioAnalyzer, and Agilent 4200 Tapestation.  Library construction is automated using PerkinElmer Sciclone instruments.  For storage and analysis of NGS data, GTAC utilizes a cluster with over 1924 processors and a combined RAM of 4.5TB.  More than 400 terabytes of redundant, fault-tolerant storage that is connected over a 10 GbE storage area network (SAN) is used for primary data storage.  The GTAC provides its customers with raw sequence data and comprehensive bioinformatic analysis for RNA-seq, ChIP-seq, targeted capture, whole exome capture and genomic sequencing using extensively validated workflows. The microarray facility was initially established in early 2002.  It is located in the Cortex building, occupies 1500 square feet, and is currently staffed by six scientists.  The facility involves itself in every step of microarray research, including custom array design, QC and labeling of both DNA and RNA, microarray scanning, statistical analysis and submission of data.  Further, the lab can support high-density protein expression analysis via the SomaScan assay.  The facility is equipped to run all microarray formats, including Illumina, Affymetrix, and Agilent.  Liquid-handling automation allows for high through-put projects.  For RNA QC the facility utilizes a Nanodrop ND-1000 spectrophotometer as well as a Agilent 2100 Bioanalyzer.  The facility currently utilizes four scanners (one Illumina iScan, two Affymetrix Genechip 3000, and one Agilent DNA Scanner).  Samples processing is automated using a Tecan Freedom EVO robot, or one of eight Affymetrix Fluidics 450 stations.  Additional automation is provided with an Illumina Beadstation auto-loading robot.  The facility has collaborated with over 500 laboratories and collaborated in work involving 25 species.  More than 20,000 expression hybridizations have been done for both Washington University investigators and outside groups.  In addition we have genotyped well over 50,000 samples using Illumina and Affymetrix technologies. The GTAC PCR facility was established in 2011 and is also located in the Cortex building, adjacent to the medical campus.  The PCR core provides researchers with several options for gene expression, copy number variation, SNP genotyping, custom amplicon generation for sequencing, and single-cell expression.  Both high and medium-to-low throughput options are available.  The facility utilizes a Fluidigm BioMark HD, Fluidigm Access Array, 10X Chromium, BioRad CFX, and BioRad QX200 ddPCR.

Access

Service available to All entities, including for-profit organizations.

Priority service for No distinctions.

Additional information:

When considering a new sequencing project, please fill out and submit the following web-form: http://https://gtac.wustl.edu/?page_id=29

Services

  • RNA-seq
  • single-cell RNA-seq
  • ChIP-seq
  • Exome-seq
  • Targeted-seq (custom capture or amplicon)
  • Whole-genome seq
  • RNA Expression (mRNA and miRNA)
  • Protein Expression (SOMAscan)
  • Genotyping (whole genome and custom)
  • Copy Number Variation

Equipment

  • Library prep automation
  • Capture automation
  • HiSeq 3000 (1)
  • HiSeq 2500 (3)
  • MiSeq (2)
  • Illumina iScan
  • Affymetrix Genechip 3000 (2)
  • Agilent DNA Scanner
  • Fluidigm BioMark HD
  • Fluidigm Access Array
  • 10x Chromium System
  • BioRad CFX
  • BioRad QX200 ddPCR

Pricing

Please contact this core directly for pricing information.


AFFILIATIONS
Institute of Clinical and Translational Sciences (ICTS)
Siteman Cancer Center (SCC)