This core specializes in small molecule screens to identify agonists/antagonist of biologically/pathologically interesting proteins, and sub- or genome-wide siRNA screens in cell functional assays. We provide support for any project that would benefit from automation.

Core Description

The Core System: The High Throughput Screening Core (HTSC) supports medium and large format screens of siRNA and chemical libraries in cell-based, chemistry, and protein-based assays. Assays may be run in 96-well and 384-well plates with detection by high content microscopy imaging, fluorescence intensity or polarization, luminescence (flash and glow), BRET, FRET, absorbance, or other readouts. A wide variety of siRNA and chemical compound libraries, molecular imaging platforms, and expertise with assay development and data analysis is available. The clean-room environment and an on-line humidified, CO2 incubator provide ideal conditions for performing cell-based assays, while the rail system and random access ensures the uniform treatment of every well across the entire screen.

High Content Screening: A GE INCell 2000 provides the platform for High Content fluorescent microscopic imaging of cells in almost any SBS format multiwell plate or slides. A CCD camera, laser autofocus, and motorized stage enable the capture and analysis of thousands of images per hour with up to 4 fluorophores and brightfield. Temperature and CO2 control allows time course imaging in live cells.

The powerful analysis software has 10 dedicated assay-specific modules for automated detection, and the flexible software, Developer Toolkit, for custom assay development. The instrumentation and software work together, automating recognition of changes at the cellular level for cell-based drug profiling, toxicological studies, and phenotypic assays to determine gene or compound function.

Access

Service available to All entities, including for-profit organizations.

Priority service for No distinctions.

Services

  • Experienced personnel work closely with researchers to develop and perform screens with siRNA or small molecules
  • Support for any research that may benefit from automation
  • Access to instrumentation, including liquid-handling, plate readers, and high-content microscopy - individual instruments may be used offline for smaller studies
  • Access to a compound collection of ~120,000 chemicals. A variety of sources and activities provides the capacity to screen relatively small bioactive libraries to develop assays or for proof-of-concept data, as well as for screening much larger libraries
  • Access to siRNA collections, including Human Phosphatase, Kinase, Druggable Genome, and Whole Genome

Equipment

  • A Beckman-Coulter Core System comprises a SCARA robot which shuttles plates between incubators, liquid-handlers, and plate readers. Versatile software schedules and coordinates the function of all instrumentation for maximum efficiency and careful control of assay parameters
  • The key elements in the platform are:
  • Biomek FX liquid handler, with both a multichannel 96 tip pod, and a Span-8 pod with 8 independent tips, is suitable for transferring and aliquotting microliter volumes to and from 96 or 384-well plates, reservoirs, tube racks, or individual micro-tubes, cherry-picking compounds, adding controls, or creating compound combinations from a file
  • The automated online humidified Cytomat incubator, with temperature and CO2 control, keeps cells at optimal condition until needed for processing
  • Bar code printing and reading stations allows plates and samples to be accurately tracked from start to readout
  • BioTek ELx405 Select plate washer has 4 solution choices, and is programmable for gentle cell washing
  • PerkinElmer EnVision plate reader with enhanced luminescence sensitivity
  • BMG FlUOstar Optima Plate Reader with injection capability for kinetic assays
  • GE INCell 2000 automated scanning epifluorescence microscope for high-content screening

Pricing

Pricing is subject to core verification

Itemized services are billed directly to the user. An estimate is made at the beginning of each assay and communicated to the PI. Upon completion of the assay, a final bill is sent to the PI. Billing to other institutions will include a surcharge.


AFFILIATIONS
Institute of Clinical and Translational Sciences (ICTS)
Department of Biochemistry and Molecular Biophysics
Center for Drug Discovery