The Optical Imaging Core (OIC) provides access to state-of-the-art optical imaging instruments for conducting studies ranging from live cells to in vivo animals. This facility is also well equipped for conducting high end spectroscopic studies. A highly successful team of NIH funded researchers, scientists and technicians under the leadership of Dr. Samuel Achilefu and Dr. Mikhail Berezin are available to discuss and provide support in conducting molecular imaging studies.

        Our priority is to provide support to NIH funded researchers making important contributions to cancer research. The Optical Imaging Core and the three sub-cores: the Microscopy Imaging, In Vivo Animal Imaging and Spectroscopy Cores are available to the Washington University community, as well as other institutions and private partners.       


Our imaging resources include:

Core Description

    We strive to provide high quality support for expediting molecular imaging studies and research.
     We have three sub-cores open for use:

    • Microscopy imaging unit
    • In vivo animal imaging unit
    • Spectroscopy unit

Each of these sub-cores is coordinated by a NIH funded faculty at the Optical Radiology Laboratory under the direction of Dr. Samuel Achilefu. Users need to obtain training for using each of resources under the direction of the respective sub-core faculty as the training and fees may change from instrument to instrument. Additional assistance for conducting individual experiments are available as well.

Microscopy Imaging Unit
Faculty: Dr. Samuel Achilefu (achilefus@mir.wustl.edu)

This core is equipped with:

  • Olympus FV1000 laser scanning confocal microscope, capable of performing 3D DIC imaging as well as 3D fluorescence imaging using Argon Ion (458nm /488nm/515nm), HeNe (543nm/633nm), and 785 nm laser excitations and corresponding fluorescence acquisition. 
  • Olympus BX51 epi-fluorescence microscope, capable of performing bright-field imaging as well as fluorescence imaging using DAPI, FITC/GFP, Cy5, Cy7, and 800 nm excitation and corresponding fluorescence acquisition. This microscope has an Olympus DP71 12.5 megapixel camera.
  • The systems are equipped with objectives from 1.25X to 100X for fluorescence imaging. Olympus provided software or custom software is available for image analysis. Free assistance is available for quantitative analysis of microscopy images.
    1.  

In Vivo Animal Imaging Unit

Faculty: Dr. Samuel Achilefu  (achilefus@mir.wustl.edu)

This core includes:

  • IVIS Lumina and IVIS 50- Xenogen In Vivo Bioluminescence Imaging Systems (BLI)
  • Pearl NIR Fluorescent Imager (LiCor Pearl) – in-vivo small animal dual- channel NIR detection for receptor & transporter targeting, structural and biodistribution imaging
  • Perkin Elmer FMT 2500 small animal fluorescence DOT system
  • Optix MX3– time-domain Diffuse Optical Imaging system, with a tunable laser excitation wavelength and filter sets for fluorescence lifetime (FLT) imaging
  • Kodak (IS4000MM), a reflectance time-domain DOT system (Advanced Research Technologies: eXplore Optix). The Kodak system performs fluorescence imaging at diverse excitation/emission wavelengths. The system also includes an enclosed 12–35 kVP X-ray source and RAD PADD radiographic imaging module for digital radiography and planar scintigraphy.
  • Custom small animal fluorescence lifetime (FLT) DOT system. Additionally, a small animal SPECT/CT, a small animal MRI scanner, and a small animal PET system are available. It also offers a small animal gas anesthesia system.  The ART system has a temperature controlled animal plate, and it performs FLT imaging using 700 nm and 780 nm excitation source. This performs time resolved FLT imaging using a Ti:Sapphire laser 780 nm excitation light.

Spectroscopy Unit

Faculty: Dr. Mikhail Berezin (berezinm@mir.wustl.edu)

This core includes:

  • VIS-NIR (300-1600 nm) spectrofluorometer from Horiba Jobin Yvon Inc. This system features four high sensitivity NIR detectors:
    • Thermoelectrically cooled R2658p PMT with sensitivity 185-1050 nm
    • Thermoelectrically cooled to -70 degree C Synapse CCD (1024×256 pixel) detector with sensitivity 200-900 nm
    • Thermoelectrically cooled H10330-45 PMT detector with sensitivity 950-1400 nm
    • Liquid nitrogen cooled linear InGaAs array detector Symphony II 512x50x1 with sensitivity 800-1700 nm. It measures FLT in spectroscopic cuvette using 295, 370, 460, 560, 648, 700, 773, 785, 820, 980 nm laser sources, and it also measures phosphorescence lifetimes.

Access

Service available to All entities, including for-profit organizations.

Priority service for Specific groups, centers or departments – see Additional Information for details.

Additional information:

Siteman Cancer Center :
Members receive priority access and study design consultation. The Optical Imaging Core is a fee based facility. Consultation with the OIC management is required before using this facility.  All users must clarify with their laboratory PI regarding the User Fees prior to reserving a time, signing up for training, or using the facility. To schedule an orientation and training session, reserve a time, or for usage instructions please email Julie Prior at priorj@wustl.edu

Services

  • 3D time-lapse confocal microscopy
  • Epi-fluorescence microscopy
  • Small animal fluorescence imaging using the multi-modal digital imaging system Kodak, ART, and custom DOT system
  • Spectroscopy, involving measuring absorption, emission, lifetime, etc. of fluorescence dyes
  • Technical support for microscopy, small animal imaging, and spectroscopic studies
  • Small animal bioluminescence imaging (BLI) with IVIS imaging systems
  • Technical support for analysis of bioluminescence imaging data

Equipment

  • IVIS Lumina- Xenogen In Vivo Bioluminescence Imaging System (BLI)
  • Pearl NIR Fluorescent Imager (LiCor Pearl) - in-vivo small animal dual- channel NIR detection for receptor & transporter targeting, structural and biodistribution imaging.
  • Xenogen IVIS 50- In Vivo Bioluminescence Imaging System (BLI) - provides sensitive and quantitative bioluminescent imaging
  • Optix MX3- time-domain Diffuse Optical Imaging system, tunable laser excitation wavelength and filter sets for fluorescence lifetime (FLT) imaging
  • Perkin Elmer FMT 2500 small animal fluorescence DOT system
  • Olympus FV1000 LSM- uses excitation of 488 nm, 543 nm, 633 nm, and 785 nm wavelengths and filter sets
  • Olympus BX51 system- with DAPI, FITC/GFP, Cy5, Cy7, and 800 nm excitation channels
  • Kodak IS4000MM system- multi-modal imaging, diverse excitation & emission channels for fluorescence imaging
  • VIS-NIR (300-1600 nm) spectrofluorometer- measure FLT of dyes in spectroscopic cuvette, using 295, 370, 460, 560, 648, 700, 773, 785, 820, 980 nm laser sources, measure phosphorescence lifetime

Pricing

Pricing is subject to core verification

For pricing information, please con​tact the faculty coordinator of the respective sub-core.


AFFILIATIONS
Molecular Imaging Center
Siteman Cancer Center (SCC)